PhanerochaeteV1, Main, Exploration, bibRecord, 000E43

The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.

Identifieur interne : 000E43 ( Main/Exploration ); précédent : 000E42; suivant : 000E44

The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.

Auteurs : T A Randall [États-Unis] ; C A Reddy

Source :

Current genetics [ 0172-8083 ] ; 1992.

RBID : pubmed:1314140

Descripteurs français

KwdFr :
- Basidiomycota (génétique), Cytosine (métabolisme), DNA restriction enzymes (physiologie), Gentamicine (pharmacologie), Méthylation (MeSH), Plasmides (physiologie), Résistance microbienne aux médicaments (génétique), Transformation génétique (physiologie), Vecteurs génétiques (MeSH).
MESH :
- génétique : Basidiomycota, Résistance microbienne aux médicaments.
- métabolisme : Cytosine.
- pharmacologie : Gentamicine.
- physiologie : DNA restriction enzymes, Plasmides, Transformation génétique.
- Méthylation, Vecteurs génétiques.

English descriptors

KwdEn :
- Basidiomycota (genetics), Cytosine (metabolism), DNA Restriction Enzymes (physiology), Drug Resistance, Microbial (genetics), Genetic Vectors (MeSH), Gentamicins (pharmacology), Methylation (MeSH), Plasmids (physiology), Transformation, Genetic (physiology).
MESH :
- chemical , metabolism : Cytosine.
- genetics : Basidiomycota, Drug Resistance, Microbial.
- chemical , pharmacology : Gentamicins.
- chemical , physiology : DNA Restriction Enzymes, Plasmids, Transformation, Genetic.
- Genetic Vectors, Methylation.

Abstract

The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.

DOI: 10.1007/BF00336850
PubMed: 1314140

Affiliations:

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (genetics)</term>
<term>Cytosine (metabolism)</term>
<term>DNA Restriction Enzymes (physiology)</term>
<term>Drug Resistance, Microbial (genetics)</term>
<term>Genetic Vectors (MeSH)</term>
<term>Gentamicins (pharmacology)</term>
<term>Methylation (MeSH)</term>
<term>Plasmids (physiology)</term>
<term>Transformation, Genetic (physiology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Cytosine (métabolisme)</term>
<term>DNA restriction enzymes (physiologie)</term>
<term>Gentamicine (pharmacologie)</term>
<term>Méthylation (MeSH)</term>
<term>Plasmides (physiologie)</term>
<term>Résistance microbienne aux médicaments (génétique)</term>
<term>Transformation génétique (physiologie)</term>
<term>Vecteurs génétiques (MeSH)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Drug Resistance, Microbial</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Résistance microbienne aux médicaments</term>
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<term>Transformation génétique</term>
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<term>Plasmids</term>
<term>Transformation, Genetic</term>
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<keywords scheme="MESH" xml:lang="en"><term>Genetic Vectors</term>
<term>Methylation</term>
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<front><div type="abstract" xml:lang="en">The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.</div>
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<Month>Mar</Month>
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<Title>Current genetics</Title>
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<ArticleTitle>The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.</ArticleTitle>
<Pagination><MedlinePgn>255-60</MedlinePgn>
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<Abstract><AbstractText>The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA of p12-6 fungal transformants, p12-6, as well as a larger plasmid, p511, were recovered in recA- E. coli strains while only p12-6 was recovered in recA+ E. coli strains. The results also showed that the cytosine methylation system has no apparent effect on the strain-dependent recovery of p12-6 and p511 in E. coli from the total DNA of fungal transformants.</AbstractText>
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The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.

The nature of extra-chromosomal maintenance of transforming plasmids in the filamentous basidiomycete Phanerochaete chrysosporium.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki